The axonal cytoskeleton down to the nanoscale

As part of the ongoing Student/Postdoc-Run Speaker Series (SPRSS), and on behalf of the Rethinking Synapses: Aspects of Synaptic Complexity and Diversity Group, Prof. Christophe Leterrier will give a talk titled "The axonal cytoskeleton down to the nanoscale."

Abstract: The intricate arborization and molecular identity of axons is maintained for decades, but must also continuously adapt to changes in the environment and modulate the activity of neurons. Axons fulfill these paradoxical demands thanks to a unique cytoskeletal organization that ensures the coordinated transport, anchoring and assembly of axonal components. In our lab, we use super-resolution microscopy to delineate and map the nanoscale architecture of cytoskeletal structures within the axon: the periodic actin/spectrin submembrane scaffold, presynaptic actin assemblies, clathrin-coated pits. We are exploring their molecular organization and functions by combining versatile labeling approaches, correlative live-cell/ super-resolution/electron microscopy and quantitative analysis that allow for high-content, nanoscale interrogation of the axonal architecture.